Mai-Otome Zwei (Japanese: 舞-乙HiME Zwei) is an official video animation (OVA) created by Sunrise as part of the Mai-HiME Project.

The four-series was premiered between November 2006 and August 2007 and is dated a year after the close of the original Mai-Otome anime, to which it is a sequel.

A manga series based loosely upon the OVA is being serialised by Champion RED.